This device consists of two independent fluidic channels that allow perfusion of culture medium for the application of biomimetic shear forces. Despite being a simple device, the potential applications are quite varied:

• Vascular endothelium models: Endothelial cells lining blood vessels are subjected to shear stress forces due to blood flow within the vessels. This shear stress is crucial for vascular homeostasis and endothelial function. Also applicable to lymphatic vessels.
• Simple epithelium models with physiological shear stress effect: intestinal, renal epithelium.
  • The epithelial cells lining the small and large intestines may be exposed to shear stress forces due to the flow of fluid and nutrients through the gastrointestinal tract. This can influence intestinal barrier function, nutrient absorption, and the immune response of the intestine.
  • Renal tubules and glomeruli are exposed to shear stress generated by the flow of fluid through the urinary system. Renal shear stress can influence glomerular filtration function and tubular reabsorption.
• Rolling and adhesion assays to study the ability of cells such as leukocytes to adhere and roll on an endothelial surface. This process is crucial for immune response and inflammation processes.
• Circulating particle assays involving immune system cells, vesicles, nanoparticles, encapsulated drugs, circulating tumor cells, bacteria, virus, under blood flow conditions.
• Biofilm studies. Flow significantly affects biofilm formation and function, influencing bacterial movement, adhesion, genetic behavior, EPS production, communication, and nutrient transport. Bacterial adhesion and growth under flow conditions better mimic their natural environment.

Compatible techniques for device use:

• High-resolution microscopy for live and fixed cells: immunofluorescence.
• Cell extraction for flow cytometry, qPCR, or western blot.
• Liquid exchange control and automation.
• Optical mapping.
• Plate reader.