The Be-Gradient Barrier-Free is a powerful microfluidic device designed to recreate 3D cell cultures in a central chamber, flanked by two adjacent lateral channels that simulate vascular perfusion without any physical barrier.

This configuration allows the generation of chemical gradients, barrier models, and vascular-like environments, all while offering full microscopy access to each compartment.

• 3D Culture with Vascular Simulation: Recreate tissue-like environments with central 3D constructs and lateral vessel-mimicking channels.
• Chemotaxis & Gradient Studies: Generate directional chemical gradients to drive and observe cell migration or spheroid invasion.
• Barrier Models: Co-culture different cell types across compartments to study tissue interfaces and barrier integrity (BBB-on-chip).
• Endothelial Modeling & Angiogenesis: Culture endothelial cells in lateral channels to explore vascularization, endothelization, or angiogenic behavior.
• Ischemia Modeling: Mimic ischemic environments by manipulating oxygen and nutrient availability under flow conditions (tumor-on-chip, ischemia-on-chip)

Gas-Controlled Microenvironments

Thanks to its gas-impermeable, it also supports gas control conditions such as hypoxia, anaerobiosis or ischemia modeling, considering key factors like cell type metabolism, nutrient/oxygen diffusion, and flow conditions.