Be-Gradient Standard

€ 400,0

Be-Gradient Barrier-Free is our device designed for electrochemical gradient application to 3D cell cultures and for creating barrier models. It is compatible with any type of optical microscopy (inverted phase contrast, confocal, fluorescence, etc). Be-Gradient consists of a cell culture chamber and two channels in direct contact with it. Changing the concentration of one element between the channels on both sides of the central chamber results in the creation of a chemical gradient. For barrier and vasculature models, the lateral channels are meant to simulate blood vessels, where endothelial cells can be cultured.

· 10 devices per box

· For special requests. Contact >

· Would you like to see more information culture models, applications, video tutorials or downloading the PDF user guide of the Be-Gradient Barrier-Free.

Go to the Be-Gradient Barrier-Free information section >

Be-Gradient Standard

€ 400,0

Be-Gradient Barrier-Free is our device designed for electrochemical gradient application to 3D cell cultures and for creating barrier models. It is compatible with any type of optical microscopy (inverted phase contrast, confocal, fluorescence, etc). Be-Gradient consists of a cell culture chamber and two channels in direct contact with it. Changing the concentration of one element between the channels on both sides of the central chamber results in the creation of a chemical gradient. For barrier and vasculature models, the lateral channels are meant to simulate blood vessels, where endothelial cells can be cultured.

· 10 devices per box

· Contact us for special requests

· For more information please Download the Technical PDF

Worldwide commercial network

We ship our products worldwide working with the best carriers.

Delivery time

Fast and efficient delivery times.

After-sales support

Live communication for assist you throughout all the process.

Worldwide commercial network

We ship our products worldwide working with the best carriers.

Delivery time

Fast and efficient delivery times.

After-sales support

Live communication for assist you throughout all the process.

Worldwide commercial network

We ship our products worldwide working with the best carriers.

Delivery time

Fast and efficient delivery times.

After-sales support

Live communication for assist you throughout all the process.

Be-Gradient Barrier-Free information

Download the Technical PDF

Study cell/spheroid invasion and/or migration creating a chemotaxis stimulus. Seed the desired cell type with single cells dispersed or in an aggregate in a biomaterial where media of different compositions can be introduced through the lateral channels.

Build 3D ischaemia models by playing with cell density, flow conditions and nutrient & oxygen availability, taking in consideration the cell type metabolism. Seed the desired cell type embedded in a biomaterial while the cell culture media is perfused through the lateral channels.

Design angiogenesis and endothelization models by culturing endothelial in close contact with other important cell types such as fibroblasts. Seed endothelial cells in the lateral channels and/or in the chamber where the direct contact between chamber and channel will allow the development of vasculature-like network.

Explore blood-brain barrier models with the culture of neurons and glial cells seeded in the chamber while endothelial cells can be seeded in the lateral channels. Similar barrier models can be performed by varying the chosen cell type.

The Be-Gradient Barrier-Free is a chip designed for 3D culture where an electrochemical gradient can be achieved. This chemotaxis stimulus is useful for cell/spheroid invasion and migration studies.

Ischaemia models can be also performed in this device, taking in consideration the cell type metabolism, cell density, flow conditions and nutrients & oxygen availability.

Another important application of Be-Gradient Barrier-Free is barrier models, where different cell types can be co-cultured, using the lateral channels as endothelial cell culture compartments. Additionally, this cell type can be explored in this device for the development of angiogenesis and endothelization models.