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BE-Doubleflow App. notes: Gut-on-chip 2
To better mimic the intestine, Gut-on-Chip (GOC) should present adequate intestinal barrier function. Absorption studies are a way to assess the entrance and permeation of substances (such as nutrients or drugs). Within adsorption studies, digestion blanks must be used as control samples to be compared with the digested ingredients or food of interest. The digesta contains digestive enzymes and bile salts at concentrations that although physiologically relevant are harmful to cells. For this reason, there are different strategies to detoxificate this digesta, among which is dilution with culture medium. However, extensive dilution of the sample will difficult the posterior analysis.
This guide compares the BE-Doubleflow (BDF) device as GOC dynamic system with static culture for the set-up parameters for absorption studies using a digestion blank.
Project: MITI2
MITI2: Gut Microbiota-Induced Tregs for Inflammatory-Bowel-Disease (IBD) Immunotherapy Last 5th of July, we took part in the kick-off meeting in Nantes of a R&D project lead by INSERM in which Beonchip will be participating in the next 4 years.MITI2 focuses on the regulatory properties of a subset of microbiota-specific TR1-like regulatory T (Treg) cells, for which an unprecedented association with the clinical outcome of patients in various inflammatory diseases has already been shown, for therapeutic use in inflammatory bowel diseases (IBD).Existing treatments are complex, with most people requiring lifelong medications, dietary and lifestyle modifications, and some requiring...
Cell recovery protocol
One of the strongest advantages of BEOnChip devices is their compatibility with microscopy which allows the monitoring of the cell culture overtime or at designated time points. However, there are several cell features that are important to evaluate, such as their genetic and/or protein expression profile. Techniques such as PCR and Western blot are not based on image acquisition but in the cell recovery and lysis. Our devices are compatible with cellular recovery and in this technical note will go step-by-step to demonstrate how to perform it.
BE-Doubleflow App. notes: Gut-on-chip 1
This guide starts to explore the advantages that BE-Doubleflow offer for the creation of a Gut on chip platform. Here, we compares the BE-Doubleflow device to an insert platform as control as in vitro platforms for gut models. Cell viability, layer formation and cell differentiation into intestinal cell phenotypes were evaluated in both systems.
Project: MICROGUT
The MICROGUT project uses new technologies to investigate the nutritional quality and interaction with the microbiota of up to 20 alternative proteins. The project recreates the microenvironment of the human colon and evaluates the real nutritional quality of proteins using in vivo and ex vivo studies. The project aims to generate standard methodologies and scientific evidence for the evaluation of protein quality and harmonization of dietary intake with human protein needs.
Project: DIAMOOC
DIAMOOC: Integrated AI Design and Engineering of 3D Bioprinted Multi-Organoids on Chip for Tumor Diagnosis and Therapy The project aims to develop an innovative colorectal cancer model that integrates a large bioprinted colorectal cancer organoid and the vascular system that nurtures it inside a microfluidic chip. This innovative model will have ground-breaking applications in pharmaceutical research and biomedicine, paving the way towards the application of in vitro models in personalized medicine starting where it is most needed, oncology. The general objective of DIAMOOC is the generation of a robust in vitro platform for the evaluation of drugs used in colorectal cancer treatment. This...
GrowDex®-T in a commercial organ-on-a-chip under active flow
The aim of this study was to establish a 3D environment using GrowDex-T, an animalfree
nanofibrillar cellulose hydrogel, in a commercial organ-on-chip device that could
endure long-term active flow. We tested different GrowDex-T concentrations in BEDoubleflow
chips and connected them to the flow from a peristaltic
pump for 7 days. During these flow experiments, the overall performance and barrier
integrity of GrowDex-T was assessed.
Intestinal epithelial monolayer formation using CACO-2 perfused with BE-FLOW and FLOW EZ™
Gut-on-a-chip models offer a powerful in vitro platform for studying the physiology and patho-
physiology of the intestine. The gut is home to a microbiome that plays an important role in
health and disease. Some of these microorganisms survive the hypoxic conditions found in the
intestine. Most commensal microbes in the digestive system are anaerobic and require low
levels of oxygen1. The BE-FLOW microfluidic device is made of a Cyclic Olefin Polymer (COP).
This material is impermeable to gases and allows the control of gas concentration within the
devices. Overall, this system offers a way to control the microenvironment of the intestinal
epithelium that is closer to the physiological state.
The importance of shear stress in biology
Shear stress plays a major role in biology. In this post we will explore the effect of shear stress on the cells of the human body and the importance of applying in apporpiately in cell culture.
History of animal experimentation and animal rights
This post is the first one on a series of posts on animal testing. Here we describe briefly the history of animal rights and the role that animal experimentation played on biomedical research.
We work daily to create the next generation of in vitro culture platforms.
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