News & Blog
BE-Doubleflow App. notes: Gut-on-chip 1
This guide starts to explore the advantages that BE-Doubleflow offer for the creation of a Gut on chip platform. Here, we compares the BE-Doubleflow device to an insert platform as control as in vitro platforms for gut models. Cell viability, layer formation and cell differentiation into intestinal cell phenotypes were evaluated in both systems.
The MICROGUT project uses new technologies to investigate the nutritional quality and interaction with the microbiota of up to 20 alternative proteins. The project recreates the microenvironment of the human colon and evaluates the real nutritional quality of proteins using in vivo and ex vivo studies. The project aims to generate standard methodologies and scientific evidence for the evaluation of protein quality and harmonization of dietary intake with human protein needs.
DIAMOOC: Integrated AI Design and Engineering of 3D Bioprinted Multi-Organoids on Chip for Tumor Diagnosis and Therapy The project aims to develop an innovative colorectal cancer model that integrates a large bioprinted colorectal cancer organoid and the vascular system that nurtures it inside a microfluidic chip. This innovative model will have ground-breaking applications in pharmaceutical research and biomedicine, paving the way towards the application of in vitro models in personalized medicine starting where it is most needed, oncology. The general objective of DIAMOOC is the generation of a robust in vitro platform for the evaluation of drugs used in colorectal cancer treatment. This...
GrowDex®-T in a commercial organ-on-a-chip under active flow
The aim of this study was to establish a 3D environment using GrowDex-T, an animalfree
nanofibrillar cellulose hydrogel, in a commercial organ-on-chip device that could
endure long-term active flow. We tested different GrowDex-T concentrations in BEDoubleflow
chips and connected them to the flow from a peristaltic
pump for 7 days. During these flow experiments, the overall performance and barrier
integrity of GrowDex-T was assessed.
Intestinal epithelial monolayer formation using CACO-2 perfused with BE-FLOW and FLOW EZ™
Gut-on-a-chip models offer a powerful in vitro platform for studying the physiology and patho-
physiology of the intestine. The gut is home to a microbiome that plays an important role in
health and disease. Some of these microorganisms survive the hypoxic conditions found in the
intestine. Most commensal microbes in the digestive system are anaerobic and require low
levels of oxygen1. The BE-FLOW microfluidic device is made of a Cyclic Olefin Polymer (COP).
This material is impermeable to gases and allows the control of gas concentration within the
devices. Overall, this system offers a way to control the microenvironment of the intestinal
epithelium that is closer to the physiological state.
The importance of shear stress in biology
Shear stress plays a major role in biology. In this post we will explore the effect of shear stress on the cells of the human body and the importance of applying in apporpiately in cell culture.
History of animal experimentation and animal rights
This post is the first one on a series of posts on animal testing. Here we describe briefly the history of animal rights and the role that animal experimentation played on biomedical research.
Organ on a chip 101
Organ on a chip for beginners. An introduction to the origins of Organ on chip technology and its groundbreaking potential to became the new paradigm in in vitro cell culture.
Tumour On Chip – From 2D models to advanced 3D microfluidic approaches
Cancer continues to be one of the leading causes of death worldwide and about one out every six deaths is caused by this disease. However, recreating realistic model of a micro-tumour remains a challenge for the scientific community.
Here, we explore the opportunities that microfluidics offers to overcome this problem.
Peristaltic pump vs pressure-based microfluidic flow control sytems for Organ on chip applications
The development of microfluidic research applied to cell culture has revealed the need to generate more accurate propulsion systems with high stability and pulseless flow. In this way, pressure pumps emerge instead of peristaltic pumps that have been widely used but create alterations in the flow rate.
This application note illustrates the effect of flow generated by a peristaltic or a pressure pump applied to a microfluidic system’s cell culture.
We work daily to create the next generation of in vitro culture platforms.
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